Interest and Overview
Our interests focus on the understanding of the molecular basis of gene regulation of diseases through translational research, specifically, but not exclusively, related to inflammation. The key aspects of our work include genome-wide gene expression analysis from human patient samples employing technologies such as RNA-Seq, Cap Analysis of Gene Expression (CAGE) or small RNA sequencing (miRNA). Our analysis goes beyond differentially expressed genes and identifies a variety of candidate elements responsible for the observed expression differences in the disease patients and the associated clinical phenotypes. Application of sequencing technology to the transcriptome previously has been utilized to uncover a range of regulatory elements and mechanisms, including regulation through transcription factors (TFs), nearby but distinct alternative promoters resulting in the same protein but employing different sets of regulatory TFs, expression of anti-sense RNA to modulate the sense-RNA and the regulatory role of expressed repeat elements and miRNAs. Subsequent functional validation studies confirm the suggested regulatory relationships.
(a) Insulin response Cell Reports
(b) Gene regulation in Severe and Controlled Asthma
(c) Complementing tissue characterization by integrating transcriptome profiling from the Human Protein Atlas and from the FANTOM5 consortium.